<a href='https://brc.riken.jp/mus/pcr05861'>Genotyping protocol -PCR-</a> The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 59, 105-107 (2010).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. Fluorescent Proteins/lacZ System B6.Cg-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb B6.Cg-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb Carrier x Noncarrier [C57BL/6NJcl]BRCではヘミ型雌の繁殖成績が悪い傾向が見られたため、主にヘミ型雄と野生型雌（C57BL/6NSlc雌）を交配して系統維持を行っている。 C (3-6 months) Carrier x Noncarrier [C57BL/6NJcl] Carrier x Noncarrier [C57BL/6NJcl] RBRC05861 Developed by Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2003. C57BL/6N background. Trangenic mice expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2. The dual fluorescent sperm show normal fertilizing ability and can be observed through uterine and oviductal walls under excitation light. Acrosome reacted sperm and acrosome intact sperm are easily distinguishable from each other by red fluorescence. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG/su9-DsRed2, in which a RFP(DsRed2) is connected to the CAG promoter with a mitochondrial import signal sequence of Atp5g1(su9). 岡部　勝 大阪大学微生物病研究所 岡部 勝先生 (2003)。C57BL/6N背景。 Developmental Biology Research Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/tbusa_mta.docx">FP license of TBUSA</A></li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Exp. Anim., 59, 105-107 (2010).<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること（https://www.ccb.osaka-u.ac.jp/en/）。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 B6-RBGS-002, B6-CAGsu9-DsRed2/Acr3-GFP Tg#RBGS002 B6-RBGS-002, B6-CAGsu9-DsRed2/Acr3-GFP Tg#RBGS002 true Masaru OKABE CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)], Discosoma sp. DsRed2 cDNA, mouse proacrosin promoter, mouse proacrosin signal peptide, Jellyfish EGFP cDNA C（3〜6か月） 精子のアクロソームにGFP, ミトコンドリアにDsRed2の蛍光タンパク遺伝子をつなげたトランスジェニックマウス。アクロソーム反応前の精子は緑と赤、反応後の精子は赤の蛍光を発する。受精時におけるイメージング等に有用である。Tgマウスは560nmの励起で全身が赤く、480nmでの励起で精子の先端が緑に光る。